Emergence of Extended Spectrum β-lactamases and Metallo-β-Lactamase Genes producing Escherichia coli among urinary tract infected women Running title/ Emergence of β-lactamases Genes in Escherichia coli .
Corresponding author: Ghofran Khudhair Ismail
Abstract
Background: In recent times, urinary tract infection (UTI) is one of the most widely recognized bacterial diseases all over the planet.The emergence of multidrug-resistant Escherichia coli is a major public health threat worldwide.Objectives: This study aims to determine the frequency of some virulence factor genes (ESBLs and MBL) and Antibiogram profile from the uropathogenic Escherichia coli isolated from Iraqi Women in Diyala. Materials and Methods: A total 250 urine specimens from women suffering from UTI symptoms. Diagnoses were then confirmed using biochemical tests , VITEK-2 system and molecular screening of β-lactamases genes were done by PCR. Results: Conducted study showed the most participants have E. coli growth 53 (21. Susceptibility testing showed that isolates had varying levels of resistance to different antibiotics, highest resistance to ampicillin ( 94.3%), amoxicillin-clavulanate (92.5%), cefotaxim(90.6%), Piperacillin -tazobactam (79.2%), Piperacillin ( 73.6%), Ceftriaxone (75.5%), Resistance patterns for the isolates was also calculated and 22(41.5%) isolates were MDR, 29 (54.7%) were XDR and the remaining 2(3.8% ) were MDS . Results of present study showed the positivity of E. coli isolates produce enzymes were (22.6%) MBL and (17.%) ESBL. The results achieved by using multiplex PCR revealed that 19(95%) isolates have TEM gene, while 7 (35%) isolates carried blaOXA gene. None of the 20 E .coli isolates had SHV gene. Results by monoplex PCR detect MBLs genes show that 4 (20%) of isolates have VIM gene, while 2 (10%) isolates carried NDM gene. Conclusion: E. coli isolates showed high resistance rates to most of the antibiotics tested. ESBL- MBLs producing E. coli showed high prevalence.
Keywords:
E.coli , UTIs, MBLs genes, ESBLs genes, PCR, Antibiotic Resistance, XDRDownloads
References
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