CD14, CD16 and CD22 as Immunological markers for toxoplasmosis
Received: 18 December 2024 \ Revised: 4 January 2025 \ Accepted: 17 January 2025 | Online: 15 October 2025
Corresponding author: Nabaa Ali Mousa
Abstract
Backround: Toxoplasma gondii is an apicomplexan parasite that may infect any nucleated cell in any warm-blooded animal and cause toxoplasmosis. Methods: blood sample were collected from couples who attended in Al-Zahraa Hospital and Baqubah teaching hospital in Diyala Province during the period from March to August 2024 from both genders (males and females) whose ages ranged between 14 to ≥ 40 years. ELISA test was conducted for all samples (376) to detect the infection and later to determine the concentration of, CD14, CD16 and CD22. Results: there was significant differences in CD14 and CD16 levels between the patients and the control group, meanwhile CD22 registered no significant difference between the studies groups. Results showed significant differences in mean level of CD14, CD16 and CD22 in IgM positive compared with IgM negative, also results showed no significant differences in mean level of CD14, CD16 and CD22 in IgG positive compared with IgG negative. Sensitivity and specificity testes were measured for the studied CD, results indicated that both CD 16 and CD22 have a high sensitivity to the test. The study showed positive correlation of IgM with the studies CD markers however CD16 has the strongest positive correlation with IgM and significant negative correlation with IgG that may indicate that CD 16 marker have a role which increased in acute infection rather than in chronic infections. Further investigations are required to better understand the role of each CD (CD14, CD16 and CD22) in the acute and chronic toxoplasmosis.
Keywords:
Toxoplasma gondii, ELISA, CD22, toxoplasmosis.Downloads
References
ASmith, N.C., Goulart, C., Hayward, J.A., Kupz, A., Miller, C.M. and van Dooren, G.G., 2021. Control of human toxoplasmosis. International journal for parasitology, 51(2-3), pp.95-121. 2. Schnittger, L., & Florin-Christensen, M. (2018). Introduction into parasitic protozoa. Parasitic protozoa of farm animals and pets, 1-10. 3. Shwab, E. K., Saraf, P., Zhu, X. Q., Zhou, D. H., McFerrin, B. M., Ajzenberg, D., ... & Su, C. (2018). Human impact on the diversity and virulence of the ubiquitous zoonotic parasite Toxoplasma gondii. Proceedings of the National Academy of Sciences, 115(29), E6956-E6963. 4. Sinai, A. P., Knoll, L. J., & Weiss, L. M. (2020). Bradyzoite and sexual stage development. In Toxoplasma gondii (pp. 807-857). Academic Press. 5. Jones, J.L. and Dubey, J.P., 2012. Foodborne toxoplasmosis. Clinical infectious diseases, 55(6), pp.845-851. 6. Elsheikha, H.M., Marra, C.M. and Zhu, X.Q., 2020. Epidemiology, pathophysiology, diagnosis, and management of cerebral toxoplasmosis. Clinical microbiology reviews, 34(1), pp.10-1128. 7. Vargas-Villavicencio, J. A., Cañedo-Solares, I., & Correa, D. (2022). Anti-toxoplasma gondii IgM long persistence: what are the underlying mechanisms?. Microorganisms, 10(8), 1659. 8. Sana, M., Rashid, M., Rashid, I., Akbar, H., Gomez Marin, J. E., & Dimier-Poisson, I. (2022). Immune response against updates RH: toxoplasmosis—some recent Toxoplasma gondii immune response. International journal of immunopathology and pharmacology, 36, 03946320221078436. 9. Bandyopadhyay, P. K., Das, N. R., & Chattopadhyay, A. (2022). Biochemical, Immunological and Epidemiological Analysis of Parasitic Diseases. Springer. 10. de Barros, R.A.M., Torrecilhas, A.C., Marciano, M.A.M., Mazuz, M.L., Pereira-Chioccola, V.L. and Fux, B., 2022. Toxoplasmosis in human and animals around the world. Diagnosis and perspectives in the one health approach. Acta tropica, 231, p.106432. 11. Actor, J.K., 2019. A functional overview of the immune system and immune components. Introductory immunology, pp.1-16. 12. Dupont, C.D., Christian, D.A. and Hunter, C.A., 2012, November. immunopathology Immune during response and toxoplasmosis. In Seminars in immunopathology (Vol. 34, pp. 793 813). Springer-Verlag. 13. Zheng, D., Liwinski, T. & Elinav, E. Interaction between microbiota and immunity in health and disease. Cell Res 30, 492–506 (2020). https://doi.org/10.1038/s41422-020-0332-7 14. Tundup, S., Srivastava, L., Nagy, T., & Harn, D. (2014). CD14 influences host immune responses and alternative activation of macrophages during Schistosoma mansoni infection. Infection and immunity, 82(8), 3240–3251. https://doi.org/10.1128/IAI.01780-14 15. Sharygin, D., Koniaris, L. G., Wells, C., Zimmers, T. A., & Hamidi, T. (2023). Role of CD14 in human disease. Immunology, 169(3), 260–270. https://doi.org/10.1111/imm.13634 16. Stern-Ginossar, N. and Mandelboim, O., 2010. Receptors on NK cells. Natural Killer Cells, pp.155 168. 17. Ereño-Orbea, J., Sicard, T., Cui, H., Mazhab-Jafari, M.T., Benlekbir, S., Guarné, A., Rubinstein, J.L. and Bouketir et al.: CD14, CD16 and CD22 as Immunological markers for toxoplasmosis … Engineering, Technology & Applied Science Research Vol. 15, No. 4, 2025 Julien, J.P., 2017. Molecular basis of human CD22 function and therapeutic targeting. Nature communications, 8(1), p.764. 18. Patir, A., Gossner, A., Ramachandran, P. et al. Single-cell RNA-seq reveals CD16- monocytes as key regulators of human monocyte transcriptional response to Toxoplasma. Sci Rep 10, 21047 (2020). https://doi.org/10.1038/s41598-020-78250-0 19. Ehmen, H. G., & Lüder, C. G. K. (2019). Long-Term Impact of Toxoplasma gondii Infection on Human Monocytes. Frontiers in cellular and infection microbiology, 9, 235. https://doi.org/10.3389/fcimb.2019.00235 20. Rahbari, A. H., Keshavarz, H., Shojaee, S., Mohebali, M., & Rezaeian, M. (2012). IgG avidity ELISA test for diagnosis of acute toxoplasmosis in humans. The Korean journal of parasitology, 50(2), 99–102. https://doi.org/10.3347/kjp.2012.50.2.99 21. Zola, H., Swart, B. (2014). CD Markers. In: Vohr, HW. (eds) Encyclopedia of Immunotoxicology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27786-3_216-2 22. Khazaei, S., Asadi, N., Majidiani, H., Foroutan, M., Khalkhali, H., Hazrati-Tappeh, K., Khademvatan, K., Yousefi, E. and Khademvatan, S., 2022. Toxoplasmosis in patients with cardiac disorders: a systematic review and meta-analysis. Novelty in Biomedicine, 10(2), pp.121-127. 23. Barzinij, K.R., 2021. Seroprevalence and risk factors of toxoplasmosis among University of Kirkuk female students. Annals of Parasitology, 67(2). 24. Shahrabi, S., Ghanavat, M., Behzad, M. M., Purrahman, D., & Saki, N. (2020). CD markers polymorphisms as prognostic biomarkers in hematological malignancies. Oncology reviews, 14(2), https://doi.org/10.4081/oncol.2020.466 466. 25. Na, K., Oh, B.C. and Jung, Y., 2023. Multifaceted role of CD14 in innate immunity and tissue homeostasis. Cytokine & Growth Factor Reviews. 26. Cui, F., Qu, D., Sun, R., Tao, H., Si, J., & Xu, Y. (2019). The Role of Circulating CD16+CD56+ Natural Killer Cells in the Screening, Diagnosis, and Staging of Colorectal Cancer before Initial Treatment. Disease markers, 2019, https://doi.org/10.1155/2019/7152183 7152183. 27. Matsushita, K., Margulies, I., Onda, M., Nagata, S., Stetler-Stevenson, M., & Kreitman, R. J. (2008). Soluble CD22 as a tumor marker for hairy cell leukemia. Blood, 112(6), 2272–2277. https://doi.org/10.1182/blood-2008-01-131987 28. Ziegler-Heitbrock HW, Ulevitch RJ (1993) CD14: cell surface receptor and differentiation marker. Immunology Today 14: 121–125. [DOI] [PubMed] [Google Scholar] 29. Landmann R, Knopf HP, Link S, Sansano S, Schumann R, et al. (1996) Human monocyte CD14 is upregulated by lipopolysaccharide. Infection and Immunity 64: 1762–1769. [DOI] [PMC free article] [PubMed] [Google Scholar] 30. Kramski M, Schorcht A, Johnston AP, Lichtfuss GF, Jegaskanda S, et al. (2012) Role of monocytes in mediating HIV-specific antibody-dependent cellular cytotoxicity. Journal of immunological methods 384: 51–61. [DOI] [PubMed] [Google Scholar] 31. Webster NL, Kedzierska K, Azzam R, Paukovics G, Wilson J, et al. (2006) Phagocytosis stimulates mobilization and shedding of intracellular CD16A in human monocytes and macrophages: inhibition by HIV-1 infection. Journal of Leukocyte Biology 79: 294–302. [DOI] [PubMed] [Google Scholar] 32. Appleby, L. J., Nausch, N., Erskine, L., Bourke, C. D., Rujeni, N., Midzi, N., Mduluza, T., & Mutapi, F. (2014). CD16 expression on monocytes in healthy individuals but not schistosome-infected patients is positively associated with levels of parasite-specific IgG and diseases, 8(8), IgG1. PLoS neglected tropical e3049. https://doi.org/10.1371/journal.pntd.0003049 33. Denkers, E. Y., & Gazzinelli, R. T. (1998). Regulation and function of T-cell-mediated immunity during Toxoplasma gondii infection. Clinical microbiology reviews, 11(4), 569–588. https://doi.org/10.1128/CMR.11.4.569 34. Al-Qadhi, B., Al-Jeboori, T. and Al-Bashir, N., 2009. Peripheral lymphocyte sub–populations analysis in hydatidosis patients with different clinical parameters. Iraqi Journal of Science, 50(1), pp.16 23. 35. Ehmen, H.G. and Lüder, C.G., 2019. Long-term impact of Toxoplasma gondii infection on human monocytes. Frontiers in Cellular and Infection Microbiology, 9, p.235. 36. Salas-Lais, A.G., Robles-Contreras, A., Balderas López, J.A. and Bautista-de Lucio, V.M., 2020. Immunobiotic and paraprobiotic potential effect of Lactobacillus casei in a systemic toxoplasmosis murine model. Microorganisms, 8(1), p.113. 37. Stites, D. P. 1976. Laboratory methods of detecting cellular immune function. In : Basic and clinical immunology. Fundenberg, H. H., Stits, D. P.and Caldwell .J. L. (eds) . pp. 318 - 322. Lange Medical Publication, California.
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